经白血病病毒转化的小鼠巨噬细胞RAW 264.7
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该细胞株建自Abelson小鼠白血病病毒诱发的肿瘤组织，是科研上最常用的炎症细胞模型之一。该细胞易于增殖，高效DNA转染力，对RNA干扰敏感，常用作转染宿主细胞和复制小鼠诺如病毒。该细胞表面sIg-、Ia-、Thy-1.2抗原阴性。据报道，该细胞建系后已不分泌且检测不到病毒颗粒，XC斑点形成实验阴性。可以抗体依赖性地分解绵羊红血球与肿瘤靶细胞，LPS或PPD处理2天后可诱导分解红血球但对肿瘤靶细胞无作用。经检测鼠痘病毒阴性。每个批次均通过本库支原体检测，结果为阴性。该细胞经过STR鉴定，结果为：①该株细胞DNA进行小鼠细胞STR分型结果显示，扩增后图谱清晰，分型结果良好：18-3：18；9-2：14.3；6-7：12,13；5-5：14,15；X-1：23.3；15-3：22.3；12-1：15.3；6-4：16.3，17.3；CSF1PO：无；vWA：无；4-2：20.3；Jarid1：X，Y。 ②该株细胞确为小鼠细胞，没有人源细胞污染。其重要性在于可为生命科学和生物技术领域的研究工作提供标准化的实验细胞资源及相关技术支撑。
This cell line was established from tumor tissues induced by Abelson murine leukemia virus, and is one of the most commonly used inflammatory cell models in scientific research. This cell line features high proliferation efficiency, robust DNA transfection capability, and sensitivity to RNA interference, and is widely used as a transfection host cell and for the propagation of murine norovirus. The cell surface is negative for sIg, Ia, and Thy-1.2 antigens. It has been reported that no viral particles are secreted or detectable from this cell line after its establishment, and the XC plaque formation assay yields negative results. It can mediate antibody-dependent lysis of sheep red blood cells and tumor target cells; treatment with LPS or PPD for 2 days can induce lysis of sheep red blood cells, but has no effect on tumor target cells. Tests have confirmed that this cell line is negative for mousepox virus. Each batch has passed mycoplasma detection in our cell bank, with negative results. STR authentication was performed for this cell line, with the results as follows: ① Genotyping of mouse cell STR loci from the cell line DNA showed clear amplified profiles and excellent genotyping outcomes: 18-3: 18; 9-2: 14.3; 6-7: 12,13; 5-5: 14,15; X-1: 23.3; 15-3: 22.3; 12-1: 15.3; 6-4: 16.3, 17.3; CSF1PO: null; vWA: null; 4-2: 20.3; Jarid1: X, Y. ② This cell line is confirmed to be of murine origin, with no human cell contamination. Its significance lies in providing standardized experimental cell resources and relevant technical support for research in the fields of life sciences and biotechnology.
