Transcript profiling of Candida albicans tor1 kinase mutants. Transcript profiling of Candida albicans tor1 kinase mutants

This study analyses the effects of truncation of the gene encoding Tor1 kinase in Candida albicans. The study involves a mutant tetO-TOR1-Del381 which has the N terminal regulator HEAT repeats deleted compared to a full length tetO-TOR1 strain and a heterozygous TOR1/tor1 control. Cell were grown in vitro in YEPD broth at 30˚C. In shut off experiments, doxycycline (30 µg.ml) was added to cultures to shut off the tetO promoter and inhibit Tor1 expression. Overall design: Study compares (i) Mutant tetO-TOR1-Del381 versus and full length tetO-TOR1, (ii) tetO-TOR1-Del381 versus heterozygous TOR1/tor1 control in the presence of doxycycline (2h and 8) and (iii) tetO-TOR1 heterozygous versus TOR1/tor1 control in the presence of doxycycline (2h and 8h). All experiments with four dye swaped replicates.