rd10 transcriptome analysis

Retinitis pigmentosa (RP) is a photoreceptor disease that affects approximately 100,000 people in the United States. There are currently very limited treatment options and the prognosis for most patients is progressive vision loss. Unfortunately, the understanding of the molecular underpinnings of RP initiation and progression is still poorly understood. However, the development of animal models of RP, coupled with high-throughput sequencing, has provided an opportunity to study the underlying cellular and molecular changes of this disease. Using RNA-Seq, we present the first retinal transcriptome analysis of the rd10 murine model of retinal degeneration. RNA-Seq on whole-retina samples from rd10, wild-type and GFP-expressing mouse retina. Three biological replicates of each.色素性视网膜炎（Retinitis Pigmentosa, RP）是一种感光细胞疾病，在美国约影响10万人。目前临床治疗手段极为有限，多数患者的预后表现为进行性视力丧失。遗憾的是，学界对RP起始与进展的分子基础仍缺乏深入认知。不过，RP动物模型的构建与高通量测序（high-throughput sequencing）技术的结合，为解析该疾病的细胞与分子层面的潜在变化提供了研究契机。本研究借助RNA测序（RNA-Seq）技术，首次针对视网膜变性rd10小鼠模型开展视网膜转录组分析。实验样本涵盖rd10模型小鼠、野生型小鼠以及表达绿色荧光蛋白（GFP, Green Fluorescent Protein）的小鼠的全视网膜组织，每组均设置3次生物学重复。