RNA-seq of hypothalamus from Zrsr1 WT and Zrsr1 mutant mice

Splicing factor ZRSR1 is known to have a role in spermatogenesis and fertility, leading its mutation to several reproductive defects in male. To investigate if Zrsr1 gene is involved in male reproductive control by the hypothalamus, we performed RNA-seq to determine the differences in gene expression, usage of isoforms and alternative splicing between the hypothalamus of WT mice and from Zrsr1mu mice.剪接因子ZRSR1（Splicing factor ZRSR1）已被证实参与精子发生与生育调控过程，其突变可导致雄性个体出现多种生殖缺陷。为探究Zrsr1基因是否参与下丘脑对雄性生殖功能的调控，我们通过RNA测序（RNA-seq）分析了野生型（WT）小鼠与Zrsr1mu小鼠的下丘脑组织在基因表达、转录本亚型使用以及可变剪接方面的差异。