consensus sequences drafted from microbial communities that were characterized with ONT 16S rRNA gene sequencing

Consensus sequences generated by CONCOMPRA, NGSpeciesID, and amplicon_sorter from (1) a synthetic bacterial community composed of 20 equally abundant bacteria (16S MOCK; https://www.atcc.org/products/msa-1002) or (2) the ZymoGut Microbiome Standard, a microbial community mimicking the human gut microbiome (Gut Microbiome Standard; https://files.zymoresearch.com/datasheets/ds1712_zymobiomics_gut_microbiome_standard_data_sheet.pdf). The first was sequenced twice, on both a R9 and and R10 flow cell (indicated by the folder structure). For CONCOMPRA, all the consensus sequences ('CONCOMPRA.all_consensus') are given as well as the non chimeric sequences only ('CONCOMPRA.noglobal_nolocalchim.consensus.sequences'). The full-length of the 16S rRNA gene was amplified with the primer set 27F and 1492R.Sequencing details as well as the data processing steps are described in: Stock, Willem, et al. "Breaking free from references: a consensus-based approach for community profiling with long amplicon nanopore data." <i>bioRxiv</i> (2024): 2024-07. ( https://www.biorxiv.org/content/10.1101/2024.07.04.602031v1 )<br>本数据集包含由CONCOMPRA、NGSpeciesID及amplicon_sorter三款分析工具生成的共识序列（consensus sequence），其来源样本分为两类：(1) 由20种丰度完全均等的细菌组成的合成细菌群落（16S MOCK；链接：https://www.atcc.org/products/msa-1002）；(2) Zymo肠道微生物组标准品（ZymoGut Microbiome Standard），一款模拟人类肠道微生物组的合成微生物群落（肠道微生物组标准品；链接：https://files.zymoresearch.com/datasheets/ds1712_zymobiomics_gut_microbiome_standard_data_sheet.pdf）。上述第一类合成群落分别在R9与R10流动池（flow cell）上完成了两次测序，相关信息已通过文件夹结构予以标注。针对CONCOMPRA工具，本数据集同时提供其生成的全部共识序列（文件命名为'CONCOMPRA.all_consensus'）以及仅包含非嵌合序列的结果子集（文件命名为'CONCOMPRA.noglobal_nolocalchim.consensus.sequences'）。本次实验采用引物对27F与1492R扩增得到16S rRNA基因的全长序列。本研究的测序细节与数据处理流程已在以下文献中详述：Stock, Willem 等人发表于*bioRxiv*（2024年7月）的研究论文《Breaking free from references: a consensus-based approach for community profiling with long amplicon nanopore data》，链接：https://www.biorxiv.org/content/10.1101/2024.07.04.602031v1。