<b>Figure 1. UL12.5 does not affect lytic replication in peripheral neurons.</b>

Titers of virus [PFU/mL]<br>1A.Viral titers from primary sympathetic neurons (SCGs) quantified at 24 hours post-infection (at an MOI of 10 PFU/cell) KOS-SPA or KOS-UL98.<br>1C.Viral titers from dermal fibroblasts) quantified at 24 hours post-infection (at an MOI of 3 PFU/cell) with KOS-SPA or KOS-UL98.<br><br>qPCR data for;<br>1B. Viral genome (vDNA) copy number, KOS-SPA (WT) or KOS-UL98 (UL12.5 null), 24 hours after infection, primary sympathetic neurons (SCGs).<br>1D. Relative abundance of mtDNA (<i>mtDLoop1</i>) transcripts measured by (RT-) qPCR 12 hours after infection with KOS-SPA or KOS-UL98, primary sympathetic neurons (SCGs).1E. Relative abundance of mtRNA (<i>mtCOX2</i>) transcripts measured by (RT-) qPCR 12 hours after infection with KOS-SPA or KOS-UL98, primary sympathetic neurons (SCGs).<br>1F. Relative <i>Ifnb</i> mRNA expression 24 hours after infection with KOS-SPA or KOS-UL98 of primarysympathetic neurons (SCGs).<br>1G. Relative <i>Ifnb</i> mRNA expression 24 hours after infection with KOS-SPA or KOS-UL98 of primarydermal fibroblasts.<br><br>1H. Representative immunofluorescence images (TIFs) of STING/TGN46 at 3 days post-transduction with either GFP or UL12.5 expressing lentiviral vector. Dataset showing STING and TGN46 (Golgi) area (px²) and calculated percentage of STING area overlap with Golgi for each condition. Measurements performed in Fiji.