The lifespan and kinetics of human Dendritic Cell subsets and their precursors in health and inflammation

Dendritic cells (DC) are specialised mononuclear phagocytes connecting innate and adaptive immunity. They comprise two principal subsets: plasmacytoid DC and conventional DC. Here, single cell RNA sequencing (scRNA-seq) was performed to analyze the expression levels of different genes in circulating human DC subsets. Overall design: Following myeloid HLA+ cell enrichment of whole blood according to the method outlined (Lubin et al., 2023). The cells were subsequently stained and sorted using a Fluoresecence-avtivated cell soritng (FACS) to isolate DC. Sorted cells were separated into droplet emulsion using Chromium X (10X Genomics) and analyzed using scRNAseq.