Gene expression profile upon miR-10b overexpression or knockdown

miR-10b precusor or miR-10b inhibitor was used to overexpress or knockdown miR-10b expression in PANC-1 cells. Microarray analysis was used to characterize the changes in gene expression profiles of PANC-1 upon miR-10b overexpression or knockdown PANC-1 cells were transfected with different microRNA oligos for 20 hr. Total RNA was extracted using Trizol method. Each expreimental condition had three biological triplicates. Twelve samples were analyzed in total.