Gene expression of mouse embryonic gonad

To elucidate the function of NANOS2 to regulate the transcriptome in embryonic male germ cells, we performed expression microarray analysis of the embyornic gonads of the Nanos2+/-, Nanos2-/- male and wild type female from E12.5 to E15.5. The Nanos2+/- and Nanos2-/- female gonads at E15.5 were analyzed as a negative control. Biological duplicates were examined at each stage, genotype, and sex for each experiment.为阐明NANOS2调控胚胎雄性生殖细胞转录组（transcriptome）的功能，我们对E12.5至E15.5阶段的Nanos2+/-、Nanos2-/-雄性胚胎性腺以及野生型（wild type）雌性胚胎性腺进行了基因表达微阵列（expression microarray）分析。本实验同时以E15.5阶段的Nanos2+/-与Nanos2-/-雌性性腺作为阴性对照，并在每个发育阶段、每种基因型及性别条件下均设置生物学重复（biological duplicates）样本进行检测。