Differential scanning calorimetry of an extant glucose oxidase from Aspergillus niger, the ancestor inferred at node N320, and the ancestor inferred at node N320 with a single amino acid change, based
aTm values were calculated from the thermal unfolding profiles presented in Figure 6. Values represent the averages ± ranges from two independent experiments per condition.
aAverage number of non-polar hydrogen atoms per amino acid residue. bTemperature at which denaturation is minimized (K). cTemperature at which growth is maximized (K). dThe lower temperature at which
Proteins from thermophilic organisms are a matter of immense interest for decades because of its application in fields like de-novo protein design, thermostable variants of biocatalysts etc. Previous