Using the tiller bases of wheat variety KN199 and its CRISPR/Cas9-based TaLA1 edited lines as materials, RNA was extracted and subjected to transcriptome sequencing.
To study genetic and molecular variability bewteen modern humans and Neanderthals, human cells were subjected to CRISPR/cas9 gene editing and next generation sequencing.
Integration of the HIV-1 provirus in the host genome ensures a persistent supply of latently infected cells. This latent reservoir is recalcitrant to antiretroviral therapy (ART) making lifelong treat
Figure S1. The pUC57-sgRNA expression vector. The sgRNA expression vector was constructed using the backbone of the pUC57 vector with a Kanamycin resistance gene. The annealed oligos were inserted bet