scRNA-seq analysis (drop-seq) of pharmacological HCN channel block with ZD7288 on rat primary cortical neuronal stem cells

We analysed transcriptomic changes of single cell transcriptomes from primary rat cortical neuronal stem cells (NSC) after pharmacologically blocking HCN channels with ZD7288 compared to untreated NSC. This drop-seq scRNA-seq aproach showed accumulation of ZD7288 treated cells in G1 phase and confirmed our previous findings from a well-based scRNA-seq approach with cell cycle arrest in G1 after HCN channel block.本研究分析了经ZD7288药理学阻断超极化激活环核苷酸门控（HCN）通道的原代大鼠皮层神经干细胞（NSC），与未处理的原代大鼠皮层神经干细胞之间的单细胞转录组转录变化。本次研究所采用的drop-seq单细胞RNA测序（scRNA-seq）技术显示，经ZD7288处理的细胞在G1期出现富集，并验证了我们此前基于微孔板单细胞RNA测序技术所获得的研究结果——即HCN通道阻断后会引发细胞周期阻滞于G1期。