RNA-seq analysis of transcriptomes of Noc4L deficient and the Nco4L sufficient Treg cells

To gain in depth transcriptional information about the Noc4L deficient and Noc4L sufficient Tregs, we performed RNA-seq analysis to compare the transcriptomes of Noc4L deficient (CD4+GFP+CD62Lhigh, cKO) and Noc4L sufficient (CD4+GFP+CD62Lhigh, Het) Tregs activated in vitro with Dynabeads for 48 hours or 72 hours. We found ~25 unique genes with differential expression between the Noc4L deficient and Noc4L sufficient Treg cells when activated for 48 hours in vitro. And ~100 unique genes with differential expression between the Noc4L deficient and Noc4L sufficient Treg cells when activated for 72 hours in vitro in two times experiment. Overall design: Transcriptional profiles of Noc4L sufficient (CD4+GFP+CD62Lhigh,Het) and Noc4L deficient (CD4+GFP+CD62Lhigh,cKO) Treg cells isolated from Foxp3CreNoc4l+/fl (Het) or Foxp3CreNoc4lfl/fl (cKO) mice then activated with Dynabeads for 48 hours or 72 hours were generated by RNA-sequencing.