Relative expression level of several genes involved in oxidative stress response and lipid metabolic pathways in Dnmt1N/+ mice and Dnmt1+/+ mice fed alcoholic or control diet.

The fold changes were compared to the expression level in the wild type mice fed control diet, which was assigned an arbitrary value of 1.qPCR analysis was performed using gene-specific primers, each sample was analyzed in triplicate and the data was normalized to 18S rRNA (n = 5). The primer sequences are provided in Table 1. Relative expression was calculated using the ΔΔCT method (15). Statistical analysis of the qPCR data was performed using the student’s 2-tailed t test. * Represents significant changes in hepatic gene expression in mice fed alcoholic diet compared to those fed control diet, ≠ denotes significant changes in the hypomorphic mice compared to wild type mice both fed alcoholic diet and # indicates gene with significantly less expression in the mutant mice compared to the wild type mice both fed control diet.