Immunological responses induced in Balb/c mice by CS/D covalently conjugated to 3M-051.

Groups on 10 Balb/c mice were immunized two doses of 2.5 µg CSP, 2 months apart in the indicated adjuvant. One month post second immunization sera were collected and analyzed. A, ELISA end point titers of mice, measured against CS/D (left) or NANP repeat peptide (right). B, IgG1 (left) and IgG2a (right) subclasses expressed as Luminex mean fluorescence intensities (MFI) at 1∶500 serum dilution. C, Percentage of total Cytokine+CD44+CD4+ T lymphocytes, extracted from vaccinated Balb/c mice and stimulated with CS/D, stained for intracellular IL-2 (left) or IFN-γ (middle) or CD8+ cells stimulated with the Kd-restricted peptide and stained for IFN-γ (right). Lines are mean with SEM.