Development of Xenopus Mucociliary Epithelium

Here we profile 10 distinct Xenopus MCE developmental stages throughout embryogenesis and characterise the expression patterns and state transition accompanying cell-fate decisions. Overall design: We harvest holoblastic blastula stage embryos (Nieuwkoop and Faber, NF stage 8), culture the explants ex vivo (referred as organoids), and profile 10 MCE development stages spanning blastula (stage 8) to gastrulation (stage 10.5, 12), neurula (stage 13, 16, 18, 21) and early tailbud development (stage 22, 24, 27), (22, 23) . After quality control (doublet removal and normalization; methods), we obtain 33,990 single-cell transcriptomes with high sampling and representation across all developmental stages.