Summary of human IgG1 and IgG2 antibody characteristics.

Characteristics of the human IgG1 (A1-D1) and IgG2 (A2-D2) antibodies used in this study including variable light chain (VL) framework type, variable heavy chain (VH) framework type, antigen (Ag) for the antibodies, as well as the calculated isoelectric point (pI). The production yield after a two-column purification process is listed in grams of antibody per liter of conditioned media, and the final product percent monomer, main peak (MP) purity, was determined by size exclusion high performance chromatography (SEC). DNP is dinitrophenyl and KLH is Keyhole Limpet Hemocyanin.