Roles of Mafb and c-Maf in MGE-derived pallial interneuron development and maturation

We utilized single-cell RNA-sequencing to identify candidate genetic targets of Mafb and c-Maf in the MGE-lineage using Nkx2.1-Cre generated neonatal wildtype (WT) and conditional Mafb/c-Maf double deletion mutant (cDKO) animals. We identified genes that likely contribute to Maf cDKO's phenotypes, which include preferential parvalbumin interneuron loss, overproduction of hippocampal interneurons and neurite outgrowth defects. Examination of differentially expressed genes between Nkx2.1-Cre generated WT and Maf cDKO at P0; We focused on the MGE-lineage interneuron subtype. >>>Raw data are not available due to file corruption<<<我们利用单细胞RNA测序（single-cell RNA-sequencing），借助Nkx2.1-Cre构建的新生野生型（WT）及条件性Mafb/c-Maf双敲除突变体（cDKO）动物，在内侧神经节隆起谱系（MGE-lineage）中鉴定Mafb与c-Maf的候选遗传靶点。我们筛选出了可能与Maf cDKO表型相关的基因，这些表型包括小白蛋白中间神经元的选择性丢失、海马中间神经元过度生成以及神经突生长缺陷。我们对出生后第0天（P0）的Nkx2.1-Cre介导构建的野生型与Maf cDKO小鼠的差异表达基因（differentially expressed genes）进行了分析，并将研究焦点集中在内侧神经节隆起谱系的中间神经元亚型上。>>>因文件损坏，原始数据无法获取<<<