RNA-SEQ analysis of E18.5 ALL Ret-positive (EGFP-labeled) male or female dorsal root ganglia neurons.

The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse (embryonic day E18.5 male or female). These can be used as a time point in comparative analysis of ontogeny of genes expressed at various developmental time points. RNA was extracted from E18.5 FACS isolated dorsal root ganglia dissected from Ret-EGFP C57BL/6J mice (EGFP knocked-in Ret locus-heterozyotes) and sequenced using Illumina Hiseq 2500 platform. Refer to protocols on the GUDMAP website under Jain.本数据集旨在通过从Ret-EGFP报告基因小鼠（胚胎发育第18.5天，雌雄不限）中分离得到的Ret阳性细胞构建基因表达谱数据，该分离过程采用荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）技术。此类表达谱数据可作为不同发育时间点基因表达个体发生比较分析的参照样本。研究人员从Ret-EGFP C57BL/6J小鼠（EGFP敲入Ret位点的杂合子）中剥离背根神经节，经FACS分离细胞后提取总RNA，并使用Illumina HiSeq 2500测序平台完成转录组测序。具体实验方案可参阅GUDMAP网站上Jain相关的实验流程。