RNA-SEQ analysis of E12.5 ALL Ret-positive (EGFP-labeled) FACSd dorsal root ganglia neurons

The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse (embryonic day E12.5). These can be used as a time point in comparative analysis of ontogeny of genes expressed at various developmental time points. RNA was extracted from E12.5 FACS isolated dorsal root ganglia dissected from Ret-EGFP C57BL/6J mice (EGFP knocked-in Ret locus-heterozyotes) and sequenced using Illumina Hiseq 2500 platform. Refer to protocols on the GUDMAP website under Jain.本数据集旨在利用从Ret-EGFP报告基因小鼠（胚胎发育第12.5天，E12.5）中收获的经荧光激活细胞分选（Fluorescence-Activated Cell Sorting，FACS）分离的转染期间重排（Rearranged during Transfection，Ret）阳性细胞，构建基因表达谱数据。该数据集可作为不同发育时间点表达基因个体发生比较分析的时间节点样本。实验过程中，研究人员从敲入增强绿色荧光蛋白（Enhanced Green Fluorescent Protein，EGFP）至Ret基因座的杂合子C57BL/6J小鼠体内剥离背根神经节，经FACS分离后提取总RNA，并使用Illumina HiSeq 2500测序平台完成转录组测序。具体实验流程可参考GUDMAP官网Jain项下的实验方案。