T-cells' gene expression after high-dose melphalan
收藏NIAID Data Ecosystem2026-03-12 收录
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资源简介：
T-cells' transduction efficacy by lentiviral transduction is diminished after high-dose melphalan. To investigate transcriptional changes regarding genes inhibiting viral entry, reverse transcription, nuclear transport and viral genome integration, we performed microarray transcription analysis. Peripheral blood mononuclear cells of patients, who were treated with high-dose melphalan and autologous stem-cell transplantation, were isolated from peripheral blood via density centrifugation and subsequently activated by CD3/28-nanomatrix (Human TransAct, Miltenyi Biotech) and Interleukin-2. After 3 days, T-cells were isolated by cell-sorting (MoFlow Astrios, Beckman Coulter), and whole-RNA was extracted by Qiagen RNEasy Kit.
创建时间：
2021-01-04
