Gene expression profile at single cell level of B cells from the Peyer's patch (PP) in the small intestine of EWSR1 WT and BKO mice

We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of PP B cells in the small intestine. PP cells from PP cells from WT and Ewsr1 BKO mice (three 8-week-old) male mice together as one sample for each group) were stained for 15 min with anti-CD45 FITC, anti-CD19 APC antibodies, and Dead marker (PI) for live-dead discrimination. Viable B cells (CD45+CD19+Dead-) were sorted on a FACSARIA sorter.