Replicative history marks transcriptional and functional disparity in the memory T cell pool

Mice received DivisionRecorder+ or unmodified OT-I T cells and were challenged with LM-OVA. During memory phase we FACS sorted memory CD8+ OT-I T cells (and sub-sorted RFP+ and RFP- cells in the case of DivisionRecorder+ cells) from spleen material and performed single cell RNA sequencing. Overall design: Spleens were collected from LM-OVA infected recipient mice, DivisionRecorder+ or unmodified OT-I T cells were isolated by FACS, after which cells were processed for scRNA-seq (10X Chromium 3' kit), and sequenced on Illumina NextSeq