pRS313, pRS314 and YCp50 are centromeric vectors while pRS424 is a 2μ vector [75, 76].TDH3 and PRC1 promoters are strong and moderate constitutive promoters, respectively. All plasmids harbor ACT1 ter
Table S1. Primers used in this studyFigure S1. Construction of an integration vector pDK017 for megL disruption. The EcoRI-SacI-digested pHS31 (pHS31-cut) was assembled with oriT fragment from pDN19 t