PCR primers used to generate chimeric GLT25D1-CEECAM1 proteins.

Plasmids used for PCR amplification are indicated for each constructs with the restriction sites used for cloning. Sequences are shown for the forward and reverse PCR primers, respectively. Bases marked in bold represent positions changed to create new restriction sites. Underlined bases mark the restriction sites used for cloning and dotted underlined bases show the partial restriction sites used for cloning. The BamHI restriction site used in MidC and MidD constructs is present at the 3′-end of the PCR product and not in the PCR primer.