MG53-PPARa Drives Stem Cell Differentiation Easing Intestinal Injury and Inflammation

Emerging evidence suggests that priming intestinal stem cell (ISC) lineages towards secretory progenitor cells is beneficial for maintaining gut homeostasis against inflammatory bowel disease (IBD). However, the mechanism driving such biased lineage commitment remains elusive. Here we show that MG53, also named as TRIM72, plays and important role in maintaining intestinal epithelium integrity against various insults-induced IBD. Specifically, MG53 deficiency leads to exacerbated IBD manifestations caused by various injuries in mice, whereas MG53 overexpression in ISCs is sufficient to ameliorate intestinal damage. Overall design: Isolated the intestinal epithelial cells from MG53-TG and WT mice by flow cytometry, on day 7 after 2.5% DSS treatment, and conducted single cell sequncing