Microsatellite data of 21 EST-SSR loci from all 503 Emmenopterys henryi samples

Aim: Several hypotheses are available to predict change in genetic diversity at expanding peripheral ranges. However, empirical evidence to test predictions of the centre-periphery hypothesis (CPH) at contracting range limits is scarce. To address this issue, we assessed spatial patterns of genetic variation, effective population size, and contemporary and historical gene flow in a widespread, Tertiary relict tree species from subtropical China. Location: Warm-temperate deciduous forests of subtropical China. Taxon：Emmenopterys henryi (Rubiaceae) Methods: We applied kernel density estimation to determine the centre of the species’ geographical range. Using microsatellite markers, we assessed genetic structure and diversity in 36 populations (503 individuals) sampled in the centre and periphery across the species’ range. We further examined both historical and contemporary gene flow. Finally, we applied coalescent methods to simulate population demography. Results: In support...