1H NMR metabolomic and transcriptomic analyses reveal urinary metabolites as biomarker candidates in response to protein undernutrition in adult rats

1H NMR metabolomics was performed on urine samples weekly and on plasma and liver samples collected at week 4 from these animals, to identify metabolites that respond to protein undernutrition. To further investigate metabolites which were fluctuated in response to protein undernutrition in terms of metabolic enzymes, hepatic mRNA microarray and quantitative PCR analyses were also performed at week 4.为鉴定可响应蛋白质营养不良的代谢物，研究团队每周采集实验动物的尿液样本开展1H核磁共振代谢组学（1H NMR metabolomics）检测，并针对该批动物于第4周采集的血浆与肝脏样本完成相关检测。为进一步探究在代谢酶层面上随蛋白质营养不良发生波动的代谢物，研究团队同时于第4周开展了肝脏mRNA微阵列及定量聚合酶链式反应（quantitative PCR）分析。