The PRV str. Becker genome (GenBank JF797219.1) was cloned as a BAC using the pBLueLox plasmid generating the BAC pBecker2 that produces the vBecker2 virus by transfection. In this way were build BAC9
Gene expression of nonsegmented negative-sense RNA viruses involves sequential synthesis of monocistronic mRNAs and transcriptional attenuation at gene borders resulting in a transcript gradient. To a
To investigate the effect of each mutation, observed in SARS-CoV-2 Omicron BA.2 compared to Omicron BA.1, on viral properties, we prepared recombinant viruses harboring each mutation. To verify the ge
