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RNA-seq of skin tissues from WT and Cd1d KO B6 mice

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To investigate the effect of iNKT deficiency in the skin, the analysis of global gene expression in the skin tissues from WT and Cd1d KO mice were performed using RNA-seq data obtained from these samples. Total RNA of ear skin tissues were isolated. RNA-sequencing was done for the transcriptomic analysis of skin from WT and Cd1d KO mice.

为探究皮肤中恒定自然杀伤T细胞(iNKT)缺失所产生的影响,本研究针对野生型(WT)与CD1d基因敲除(Cd1d KO)小鼠的皮肤组织全局基因表达展开分析,所用数据来源于上述样本的RNA测序(RNA-seq)结果。实验过程中,研究人员首先分离了耳皮肤组织的总RNA,随后完成RNA测序以开展两组小鼠皮肤的转录组学分析。

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