Effects of FOXN1 or AFF4 deficiency on gene expression in the skin

Loss-of-function mutations in Foxn1 or Aff4 cause similar defects in skin morphology. To determine whether these similar phenotypes result from similar changes in gene expression, skin from wild-type, Foxn1-null, or Aff4 conditional knockout (cko) mice was analysed by RNA-seq at a time when the animals were attempting to grow their first hair coats (P6). RNA preparations from Foxn1-null or Aff4-cko mutants were compared to RNA preparations from their respective wild-type littermates.Foxn1或Aff4基因的功能丧失型突变可引发相似的皮肤形态学缺陷。为探究此类相似表型是否源于相似的基因表达变化，研究人员在小鼠尝试长出第一茬被毛的时期（出生后第6天，P6），对野生型（wild-type）、Foxn1基因敲除（Foxn1-null）或Aff4条件性敲除（cko）小鼠的皮肤样本开展了RNA测序（RNA-seq）分析。研究人员将Foxn1-null或Aff4-cko突变体的RNA样本，与其各自对应的野生型同窝小鼠的RNA样本进行了比对分析。