mRNA transcriptome analysis of hypoxia with shSETDB1 in hela cells

Purpose: The goals of this study are to compare NGS-derived Hela with shSETDB1 and shcontrol in hypoxia transcriptome profiling (RNA-seq) to quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods Overall design: Methods: mRNA profiles of shControl hela and shSETDB1 with or hypoxia (1% O2) for 12 hours were generated by deep sequencing, in duplicate. GSEA was performed, qRT–PCR validation was performed