Pearson’s correlation coefficients of internal FVIII in HUVECs concurrently stained with β-actin and Factor H.

Pearson’s correlation coefficients (PCC) were measured in HUVEC images stained concurrently for FVIII and either with β-actin or with Factor H, using the antibody pairs described in the legend for Fig 6. Data comparing FVIII either with β-actin or with Factor H was analyzed from 12–14 images from 3–4 experiments of FVIII detection with each of these proteins. aThese values are below the PCC values >0.5 that signify detection correlation. [26] bP<0.001 compared to PCC values obtained for FVIII detection correlated to VWF detection in HUVECs at both 60× and 100× (S2 Dataset). Pearson’s correlation coefficients of internal FVIII in HUVECs concurrently stained with β-actin and Factor H.