MicroRNA expression in CUL4B-depleted LNCaP cells and control. MicroRNA expression in CUL4B-depleted LNCaP cells and control

We examined a global miRNA expression in CUL4B-depleted LNCaP cells to identify miRNA(s) regulated by CUL4B. Overall design: We carried out miRNA expression profiling in LNCaP cells with siRNA transfection vs. Negative control. The interference efficiency was verified by Real-Time PCR (qPCR) and western blot. Differentially expressed genes were identified based on standard and advanced quality control metrics (Fold change >2.0 and P-values <0.05).