Determining sex-specific RNA interactors of maternal Transcription factor CLAMP in Drosophila cell lines
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We performed RNA-protein interaction study iCLIP, i.e single nucleotide resolution uv crosslinking and immunoprecipitation for DNA binding transcription factor CLAMP in different cellular fractions (Chromatin fraction, Nucleoplasmic fraction, Cytoplasmic Fraction) to explore sex-specific RNA binding properties of CLAMP in female (Kc) and Male (S2) cells Overall design: CLAMP antibody used to immunoprecipitate RNA targets in different cellular fractions from UV crosslinked Kc (female) and S2 (male) cell line samples. Bound RNA is extracted and sequenced to determine CLAMP RNA targets and RNA sequence motifs for binding. Four replicates for each fraction and cell type performed. As control two replicates of UV treated, no antibody controls performed for each fraction and cell type.
