Transcription profiling by high throughput sequencing of pancreatic beta cells from wild type and Abcc8 knockout mice

Abcc8 (sulfonylurea receptor 1, SUR1) is an essential part of the ATP-sensitive potassium channel necessary for coupling energy metabolism and membrane potential. In this study we assessed the impact of eliminating Abcc8 on islet morphology, beta-cell identity, and gene expression. Beta cells were isolated by FACS from 60 day old mice based on expression of a MIP-GFP transgene.Abcc8（磺酰脲受体1，SUR1）是ATP敏感性钾通道的关键组成部分，该通道是耦联能量代谢与膜电位所必需的。本研究评估了敲除Abcc8对胰岛形态、β细胞特性以及基因表达的影响。研究人员基于MIP-GFP转基因的表达，通过荧光激活细胞分选（FACS）从60日龄小鼠体内分离得到β细胞。