Intracellular delivery of gold nanoparticles assisted by monolayer functionalisation with HA2/TAT peptides (Figure 1 dataset)

HeLa cells were incubated with or without nanoparticles for 3h, fixed and imaged by photothermal microscopy. When present nanoparticles were of a 5 nm diameter at a concentration of 500 nM. Images are two-plane TIFF images of the nanoparticles uptake by HeLa cells. The first image plane is the brightfield image, and the second image plane is the photothermal image. Cells_only_[1-4].tif are images of cells incubated without nanoparticles. 100%_capping_peptides_[1-4].tif are images of cells incubated with nanoparticles coated with a 4:1 ratio of CALNN : CCALNN-PEG peptides (capping peptides). 10%_dHA2_[1-4].tif are images of cells incubated with nanoparticles coated with 10 % CCALNN–dHA2 peptides and 90% capping peptides. 1%_Tat_[1-3].tif are images of cells incubated with nanoparticles coated with 1 % CALNN–TAT peptides and 99% capping peptides. 10%_dHA2_&_1%_Tat_[1-4].tif are images of cells incubated with nanoparticles coated with 10 % CCALNN–dHA2 peptides, 1 % CALNN–TAT peptides and 89% capping peptides.