Impaired T cell and neoantigen retention in time serial analysis of lung cancer patients treated with TIL cell therapy

We mapped TIL clones into individual transcriptomes and found that neoantigen-reactive clonotypes expressed a dysfunctional program and lacked stem-like features among patients who lacked clinical benefit. Overall design: Isolated and cultured tumor infiltrating T cells from NSCLC were collected and analyzed using single cell transcriptome sequencing paired with single cell TCR sequencing