Export of an Rv0132cSS–'BlaC<sup>1</sup> fusion protein is dependent on the Tat pathway.
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1'BlaC = truncated BlaC lacking its native signal sequence. 2All strains were resistant to 20 µg/mL kanamycin due to the vector resistance marker. The presence (+) or absence (−) of carbenicillin resistance was determined by colony growth on LB–agar plates plus 20 µg/mL kanamycin and 50 µg/mL carbenicillin for M. smegmatis and 7AGT plates plus 20 µg/mL kanamycin and 50 µg/mL carbenicillin for M. tuberculosis. See materials and methods for additional experimental details. 3Carbenicillin resistance was determined after 4–7 days. 4Carbenicillin resistance was determined after 21 days.
创建时间：
2012-10-22
