Microarray-based gene expression analysis of DMBA and high fat diet-induced hepatocellular carcinoma in mice

The gene expression in the liver tumor tissues or non-tumor tissues of DMBA-treated, high fat diet-fed mouse livers compared with those of DMBA-treated, normal diet-fed mouse liver tissues were successfully analyzed through the expression profiling of a total of 23473 genes in mouse livers (three tumor bearing livers vs two normal mouse livers). In this study, mRNA samples from three tumor bearing high fat diet-fed livers and two normal diet-fed livers were induvially used to acquire gene expression.