Genome-wide expression profiling of acute promyelocytic leukemia NB4 cells treated with all-trans-retinoic acid. Genome-wide expression profiling of acute promyelocytic leukemia NB4 cells treated with all-trans-retinoic acid

Acute promyelocytic leukemia (APL) is a hematological disease characterized by a balanced reciprocal translocation that leads to the synthesis of the oncogenic fusion protein PML-RARα. APL is mainly managed by a differentiation therapy based on the administration of all-trans retinoic acid (ATRA) and arsenic trioxide (ATO). However, therapy resistance, differentiation syndrome, and relapses require the development of new low-toxicity therapies based on the induction of blasts differentiation. Here, we performed a high-throughput gene expression profile of the maturation inducible APL cell line NB4 untreated or exposed to ATRA. Overall design: We performed a whole mRNA transcriptome analysis by Affymetrix microarray of NB4 cells exposed to 1 µM ATRA for 120h or only to DMSO as control (Ctrl). Two biological replicates were performed for each condition.