Transcriptomic analysis of ETV6/RUNX1 KO lymphoid cell line generated by CRISPR/Cas9 showed a distinct expression signature and a deregulation of its downstream signaling genes

The gene expression profile of E/R KO clones versus REH cells and controls clones, analysed by total RNA-sequencing, showed a total of 342 genes differentially expressed (q<0.05), 182 upregulated and 160 downregulated. The heatmap of the top50 of the most deregulated genes according to fold change (FC) values showed a distinct expression signature of E/R KO clones as compared with REH cells and control clones Purpose: Analyse the gene expression profile of ETV6/RUNX1 KO clones versus REH cells and controls clones by total RNA-sequencing to elucidate the functional effects of fusion gene abrogation in ALL cells.