ReNcell differentiation: Total proteomics during differentiation with small molecule perturbagens. Dataset 3 of 3

ReNcell-VM neuroprogenitors were differentiated by growth factor (EGF and FGF) removal for 15 days in culture, giving rise to dopaminergic neurons, astrocytes, and oligodendrocytes. Drugs (Mevastatin and Kenpaullone) previously screened for their promoting effects on neural differentiation or DMSO control were added to the growth factor free maintenance media during the course of differentiation. Cells were collected at 10 time points and processed for total proteomics by liquid chromatography mass spectrometry (LC/MS) using 10-plex tandem mass tag (TMT) labelling to achieve deep quantification of proteome dynamics.