Hypoglycaemic stress: sampling 60 min after treatment.

Blood samples were collected 60 minutes after insulin injection. The blood glucose level decreased (treatment p<0.01) and the ACTH level increased in the treated groups (treatment p<0.01). The corticosterone level increased after insulin-induced hypoglycaemia, but this elevation was significantly lower compared to adults (age p<0.01; treatment p<0.01 age × treatment p<0.01; treated adults vs. treated juveniles p<0.01). The aldosterone level was elevated after insulin injection in both age groups (treatment p<0.01). *p<0.05, **p<0.01 vs. Control; ##p<0.01 vs. Adult.