Quantitation of specific mRNA changes in <i>(iso)4E-1</i> Arabidopsis plants.

amRNAs significantly (p<0.001) changed (±1.5 cutoff) at both polyribosomal (P) and non-polyribosomal (NP), but not at total (T) RNA levels were considered for quantitation by real time RT-PCR (qRT-PCR). Selection was made randomly. bReal time RT-PCR was performed with a different biological sample at the same time point as microarrays (15 day-old seedlings). The results were normalized according to eIF4A control gene and show fold of change of the mRNA level in NP and P separately. n.d.: not detected. l.c.: lack of correspondence between microarray and qRT-PCR measurements.