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Identification of cellular CDK9 substrates using an analog-sensitive cell line

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NIAID Data Ecosystem2026-03-11 收录
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Analog-sensitive kinase technology allows for specific inhibition of a given kinase. Here we utilize an analog-sensitive CDK9 cell line to determine the impact of CDK9 inhibition on the phospho-proteome of human cells. Specifically, we aim to identify substrates of CDK9 in a quantitative way using stable isotope labeling by amino acids in cell culture (SILAC) followed by LC-MS/MS.

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2019-11-11
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