RIME of PbAP2-TR and PbMORC at the trophozoite stage

To explore the interaction-partners of PbAP2-TR and PbMORC in the asexual blood stage of Plasmodium berghei, rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) was performed, which is a combination of chromatin immunoprecipitation and liquid chromatography tandem-mass spectrometry. Parasites expressing GFP-fused PbAP2-TR and PbMORC were each generated from the Cas9-expressing parasite, and pbap2-g was then disrupted. Whole blood was harvested from mice infected with this transgenic parasite at 12 h post infection,and the parasites were then subjected to RIME experiments using anti-GFP antibody. Same procedures were performed for wild-type parasites as a control.