The transformed fluorescence ratio k' is the transformation of the ratio of two fluorescence intensities when one fluorescence reaches its inflexion point.RAF: Predefined Resistance allele frequency (
The target was human genomic dsDNA, wild-type for CFTR. The 25-mer probes were 2789+5G→A-WT25C (wild-type) and 2789+5G→A-MUT25C (mutant). 500 nM YOYO-1 was present in each sample. TAF indicates Triple
