Whole-genome sequencing data and BSA-seq data used to map mutations altering expression noise of a yeast gene

NGS data used in the research article entitled "Trans-acting mutations reveal non-nuclear modulators of both intrinsic and extrinsic gene expression noise in a eukaryote." Gene expression regulation is a stochastic process that can be modified by mutations not only in a deterministic manner but also in a probabilistic way, for example by changing the extent of cell-to-cell variability in gene expression (also called “gene expression noise”) without necessarily changing the expected (mean) expression level. We applied genetic mapping on yeast strains (Saccharomyces cerevisiae) generated by random mutagenesis to identify mutations that changed the expression noise of a reporter gene in the nuclear genome regulated by the yeast TDH3 promoter. Our mapping strategy relied on two types of NGS data archived in this study: 1) Illumina sequencing of the genomes of five yeast strains with random mutations generated by chemical mutagenesis (MiSeq standard V2 2 x 250); 2) Deep sequencing of genomic DNA from bulks of segregants sorted based on their fluorescence level (BSA-seq data for 5 x 3 bulks ).