Pseudomonas aeruginosa PA14 transcriptome during co-culture Aspergillus fumigatus

Pseudomonas aeruginosa monocultures and co-cultures with Aspergillus fumigatus during growth in SCFM2 were harvested in the stationary phase of growth (triplicates) by aspirating the contents of the petri-dish well into a 15 ml falcon tube (passed over a 40 µm cell strainer) and combining this with an equal volume of RNA-later. Cultures stored in RNAlater were pelleted, resuspended in 1 mL RNA Bee, and RNA was extracted as described previously.